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Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity
時間 2021-07-14
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(NeuroToxicology 3區,港大 25) 1.簡介 SY5Y是一種多巴胺能神經元細胞系。RA的分化表現爲神經突廣泛生長,神經元細胞核、神經元特異性烯醇化酶、突觸球蛋白、突觸相關蛋白-97表達增加,分化抑制劑-1表達減少。 細胞培養:接種後48小時內,10%熱滅活牛血清的培養基培養。之後換3%熱滅活血清,10μMRA培養起七天。做蛋白印跡時,按2105cells/孔的密度接種到6孔板中。
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