由於把雙端的read須要判斷方向,因此第一步的時候就已經用根據建庫的方式,duTP建庫第一條鏈作反向互補,在read後面加上/1,第二條鏈加上/2,SMART建庫的時候,第二條鏈作反向互補加上/1,第二條鏈加上/2。由於作這個轉化的時候在read名字後面多了一個space號,因此出現了上面相似的問題spa
@E00511:189:HC3TYCCXY:5:1101:3051:1959/1
GGGCATGGGATTTACTTATTCAGCAAAGGGTTGCCTTTACCACCCAGAAGGATTTTGGAAGAGAGGCCAGAGAGATAAGATGGGCTCATGCTCAGAGGACTTTGCATGGATTACAACCACCAGACACAAAACTGTTTGGTGACAGAA
+
<F7FJFFJJJAJJJFJJJJJJJJJFAJJJJJJJJJJJJJJJJJFFFFJJJJJA<A<JJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJAJJJFJJJJJJJJFAAJFJFJJJ-FJFJJJ-A-FJJJJJJJJJJJJJJJJFJJFJJJJJJ
@E00511:189:HC3TYCCXY:5:1101:3133:1959/2
GGTGTAGCAGGAACTGGACCGTCCTCCGCGAGGTCTGATAATGCCTCGATTGGTGTATTGCCTGCTAATTTGTCTTCTAATAGAGAGAGCAAGCCCATTTCAGAGAACCCGGAAGAAGAGAACGATGGAGTCCGGGATATTCATGTA
+
FFJAJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFJJJJJJJJJ7AJJFAFFJJF-FJJJJJJJJ<JJJJJJAJJJJFJJJJJJJJJJJ<JJJFJJJFJJJJJJJJJJFJJJJJJJFAFJJJJJJJJJJJFJJJJJJJJJFJJJJJJ